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KMID : 0858620060100020135
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Korean Journal of Audiology
2006 Volume.10 No. 2 p.135 ~ p.140
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Audiologic Study in Guinea Pig after Administration of PAI-1
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Kim Beom-Gyu
Park Il-Seok
Kim Yong-Bok
Kang Hun-Hee
Chung Jong-Woo
Jeon Sea-Yuong
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Abstract
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Plasminogen activator inhibitor type 1 (PAI-1) is an important regulator in the degradation of extracelluar matrix and fibrinolysis which inhibits thrombolysis in the vascular system. PAI-1 is normally secreted by endothelial cells, vascular smooth muscle cells, hepatocytes, platelets and adipocytes, with the majority of the circulating PAI-1 contributed to by adipose tissue. In type II DM, coronary artery diseases and peripheral vascular diseases, however, many studies report that plasma PAI-1 levels are elevated. In this study, we investigated the physiologic changes of cochlea after administration of PAI-1 in guinea pigs to find out the effect of PAI-1 on the microcirculation of cochlea. Guinea pigs with normal Preyer’s reflex were divided into a control group and treatment groups receiving 10, 20, 40 μg/ml of PAI-1. Using auditory brainstem response (ABR), the hearing threshold was measured during the four weeks after placement of saline and PAI-1 on the round window membrane (RWM). Endocochlear potential (EP) was checked through endolymphatic space at the 2nd turn of cochlea. The ABR threshold was increased from mild to moderate at 30 minutes for the majority of the groups, but they began to recover at 1-3 days (p<0.05). The hearing threshold was not proportional to it’s concentration. EP was significantly decreased in most PAI-1 groups in contrast to the control group (p<0.05). From these results, we can conclude that PAI-1 plays a role in the alteration of microcirculation of cochlea, and that it leads to transient hearing loss. Our studies suggest that PAI-1 is involved in part in the mechanism of inner ear disease that results from the disturbance of microcirculation.
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KEYWORD
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Plasminogen activator inhibitor-1, Hearing threshold
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